Welcome to ZenklusenLab
Our laboratory studies the regulation of gene expression at the single cell level. Our main focus is to use microscopy to determine how individual cells execute and coordinate the different steps along the gene expression pathway, allowing the controlled expression of their genes. We therefore combine single molecule techniques and quantitative analysis methods with genetics and biochemistry to gain better understanding of the general rules governing the expression of genes in complex systems, using yeast and mammalian cell models.
We are particularly interested in two steps of the gene expression pathway, transcription regulation by non-coding RNAs and RNA transport/localization. Read More ...
Tracking mRNAs on their way to the cytoplasm
In our recent papers in the Journal of Cell Biology, we used real time particule tracking to show how mRNAs find their way to the cytoplasm.
Expanding the RNA imaging toolbox
Combing MS2 and PP7 RNA tags allows now for simultanous detection of multiple RNA species in living cells. Hocine, Raymond et al., nautre methods
Vatsi's first prepublication investigating mRNP organization in cells and challenging the closed loop translation model. BioRxiv.
Collaboration with the Botton lab published in J Gen Virol.
Vatsi presenting this project on mRNP organization at the Riboclub meeting 2017.
Kevin is joning the lab as a Masters student. Finally we have a hockey fan in the lab!
Welcome Zacharie and Rhushikesh.
Samir and Cornelia's eRNA paper published in NAR.
Collaborative paper with the Rout, Sali and Chait labs published in Cell.
New plasmids for PP7 tagging from Saroufim et al., JCB 2015 are now available on Addgene.
Welcome Xuedong, MITACS student from Jilin University !
'JCB - In Focus' on our papers : mRNAs scan themselves out .
Mark-Albert's paper published in JCB .
Daniel talking at Riboclub 2015 .
Welcome Srivathsan !
Congrats Samir for receiving the 'Simon-Pierre-Nöels' prize!
Welcome Denis, Samy and Kevin, the 2015 summer team.
Cornelias's paper in PNAS is online.
Congrats Mark-Albert for your Master thesis!
Finally. Zenklusenlab Photo Stream is up.
Welcome Pierre !
Congratulation Sandra for completing your Master thesis.
Daniel talking eRNAs at Long-Range Genome Organization and Transcription Dynamics conference at Janelia Farm.
Daniel at Stochasticity in Biochemical Reaction Networks workshop in Banff.
Samir's methods chapter in Methods Mol Bio is online.
Zenklusenlab plasmids now available on Addgene.
Samir's paper published in nature structural & molecular biology - Bimodal expression of PHO84 is modulated by early termination of antisense transcription.
Samir and Daniel at RNA 2013 in Davos, Switzerland.
Regulating RNA turnover - Vincent's paper published in Cell A Nutrient-Responsive Pathway that Determines M Phase Timing through Control of B-Cyclin mRNA Stability
Welcome Melissa and Emmanuel!
Pascal's first paper published in Nature methods Single-molecule analysis of gene expression using two-color RNA labeling in live yeast
EMBO | EMBL Symposium The Complex Life of mRNA Daniel's presenting Samirs project
Riboclub meeting 2012 Congratulation Samir for winning a travel award!
We are on TV! CBS-Explora
Correntin and Michael are leaving. Good luck at Columbia/Medical school.
Daniel presents Samirs data at the EMBO meeting: Gene Transcription in Yeast, from Mechanisms to Gene Regulatory Networks
Summer sudents start. Welcome Frédérique, welcome back Zack!
Check this out: Our MDN1 FISH image is the first picture that appears on the Saccharomyces genome database website! http://www.yeastgenome.org/
mRNA export review is online. 'To the pore and through the pore: A story of mRNA export kinetics'
Tatjana's FISH protocol is online in nature protocols. Single-mRNA counting using fluorescent in situ hybridization in budding yeast.
The first parts of the new microscope are in :-)
Happy New Year everyone
New web site is up ! Thanks Sandrine !
We are located at the Université de Montréal in the Département de Biochimie and are funded by the Canadian Institutes of Health Research (CIHR), the Natural Sciences and Engineering Research Council of Canada (NSERC), Fonds de recherche Santé Québec (FRSQ) and the Canada Foundation for Innovation (CFI).